Understanding how organisms respond to habitat patterns within seascapes is fundamental for designing effective conservation strategies. There has been a recent increase in studies applying the conceptual framework of landscape ecology in the marine realm, reporting how the composition (e.g. type) and configuration (e.g. size, proximity) of habitats within coastal seascapes relate to the distribution of species and ecological processes. However, few studies have investigated how temperate reef fish respond to seascape patterning. This study employed a multi-scale approach to explore how habitat patterns explain the distribution of temperate reef fish within Jervis Bay Marine Park. Our results suggest the connectivity (distance) and size of reef and seagrass habitats is an important predictor for the distribution of temperate reef fish, with a more abundant and diverse fish assemblage found on reefs close to large seagrass beds. The influence of adjacent seagrass beds on temperate reef fish is likely due to many species using seagrass as nursery or foraging areas. These findings demonstrate the critical need for marine park design to consider the spatial arrangement of multiple habitat types to maximise conservation outcomes.